Exploring the mechanism of Xihuang Pill's anti-hyperplasia of mammary glands effect based on UPLC-Q-orbitrap-MS technology / 药学学报

Based on UPLC-Q-orbitrap-MS and biological network analysis tools, the mechanism of Xihuang Pill in improving hyperplasia of mammary glands was systematically analyzed. The rat model of hyperplasia of mammary glands was established by intramuscular injection of estradiol benzoate and progesterone. LC-MS tissue metabolomics was used to explore the key metabolites and metabolic pathways of Xihuang Pill in improving hyperplasia of mammary glands in rat. The network analysis of the key metabolites regulated by Xihuang Pill was carried out by integrating biological network analysis tools, focusing on the key metabolic pathways, and exploring the potential targets of Xihuang Pill to improve hyperplasia of mammary glands. Compared with the control group, there were significant differences in the content of 49 differential metabolites in the tissues of the model group (P < 0.05). Xihuang Pills could significantly call back 17 metabolites such as L-alanine, threonine, indole-3-carboxylic aldehyde, lysine, arginine, alanylleucine, glycyltyrosine, γ-glutamyl leucine, vitamin B3, serine leucine, threonine leucine, isoleucine glutamic acid, γ-glutamyl tyrosine, decanoyl-L-carnitine, uric acid, leucylleucine, S-adenosyl-methionine. Further network analysis and literature research on the key metabolites regulated by Xihuang Pills showed that the AGE-RAGE signaling pathway may be one of the important pathways for Xihuang Pills to improve hyperplasia of mammary glands. STAT3, MAPK1, EGFR, CASP3, CASP8, PRKCA and JUN in the AGE-RAGE signaling pathway may be potential targets for Xihuang Pills to improve hyperplasia of mammary glands. The animal experiment operations involved in this paper follow the provisions of the Animal Ethics Committee of Gansu University of Traditional Chinese Medicine and pass the ethical review of animal experiments (approval number: 2022-705).

Mechanism of blood-activating and mass-dissipating Chinese patent medicine against hyperplasia of mammary glands and use with other medicine: a review / 中国中药杂志

Caused by endocrine disorder, hyperplasia of mammary glands(HMG) tends to occur in the young with increasing incidence, putting patients at the risk of cancer and threatening the health of women. Therefore, the prevention and treatment of HMG is attracting more and more attention. Amid the modernization of traditional Chinese medicine(TCM), many scholars have found that Chinese patent medicine has unique advantages and huge potential in treatment of endocrine disorder. Particularly, Chinese patent medicine with the function of blood-activating and mass-dissipating, such as Xiaojin Pills and Xiaozheng Pills, has been commonly used in clinical treatment of HMG, which features multiple targets, obvious efficacy, small side effect, and ease of taking and carrying around. Clinical studies have found that the combination of Chinese patent medicine with other medicine can not only improve the efficacy and relieve symptoms such as hyperplasia and pain but also reduce the toxic and side effects of western medicine. Therefore, based on precious pharmacological research and clinical research, this study reviewed the mechanisms of blood-activating mass-dissipating Chinese patent medicine alone and in combination with other medicine, such as regulating levels of in vivo hormones and receptors, promoting apoptosis, inhibiting angiogenesis, improving hemorheology indexes, enhancing immunity, and boosting antioxidant ability. In addition, limitations and problems were summarized. Thereby, this study is expected to lay a theoretical basis for the further study and clinical application of blood-activating mass-dissipating Chinese patent medicine alone or in combination with other medicine against HMG.

Research progress on traditional Chinese medicine compounds combined with external therapy in treatment of hyperplasia of mammary glands / 中国中药杂志

Hyperplasia of mammary glands is a benign breast disease with disordered breast structure. Nowadays, the incidence rate of breast hyperplasia in women is increasing year by year, and the etiology is related to the imbalance of estrogen and progesterone in the body. The symptoms include breast pain, breast nodules, or nipple discharge, which can develop into breast cancer in the context of psychological pressure. Therefore, it is timely and effectively necessary for people to treat the symptoms. At present, traditional Chinese medicine(TCM) often treats breast hyperplasia by oral drug, external application, acupuncture, moxibustion, and massage, while western medicine often uses hormone therapy or surgery. TCM can regulate hormone levels to treat breast hyperplasia. Acupuncture, moxibustion, and other methods can stimulate acupoints to reduce breast lumps. However, since TCM is easy to produce hepatorenal toxicity after long-term use and simple external treatment is slow to take effect, rapid and effective treatment is difficult to be achieved. Although western medicine can inhibit the disease, it is easy to produce toxic and side effects if taken for a long time. In addition, surgery can only remove the focus and the recurrence rate is high. Some studies have found that the combination of oral and external use of TCM compounds has a significant effect, with mild toxic and side effects, few adverse reactions, and a low recurrence rate. Based on the relevant literature in recent years, this article reviewed the combination of oral and external treatment of TCM in the treatment of hyperplasia of mammary glands, discussed the effectiveness, clinical evaluation indexes, and mechanism, and pointed out the existing shortcomings to explore a comprehensive therapy worthy of clinical application.

Mechanism of Prunellae Spica Against Breast Diseases： A Review / 中国实验方剂学杂志

Prunellae Spica is the dry ear of the labiaceae plant Prunella vulgaris， which is a traditional medicine and food plant with many functions. Prunellae Spica can clear liver-fire， improve eyesight， disperse knot detumescence. It owns hot and bitter flavors and cold property. It goes to the liver， gallbladder meridian， and is a kind of commonly-used antifebric. Prunellae Spica has been used in the treatment of mammary gland diseases since ancient times.The mammary abscess， mammary nodules， mammary carcinoma of traditional Chinese medicine all belong to breast disease， and the liver meridian is most closely related to these diseases. With the development of social life， breast disease has gradually become the most primary health problem for women. Modern pharmacological studies show that Prunellae Spica contains terpenoids， phenolic acids， flavonoids and other biological active components， which have anti-inflammatory， antibacterial， hormone regulation， anti-tumor and other effects. Prunellae Spica inhibits the p38 mitogen-activated protein kinase （p38 MAPK）/nuclear factor kappa-B （NF-κB） pathway to play an anti-mastitis role， interferes with the effects of estrogen receptors or regulates lipid levels to treat breast hyperplasia， and treats breast cancer through promoting the apoptosis of breast cancer cells， inhibiting the migration of breast cancer cells， regulating the division of breast cancer cells and other ways. While referring to the relevant literature， it was found that Prunellae Spica often exerted pharmacological effects through multi-channels and multi-target regulation， but most of the studies did not specify the specific target of its effect， which needs further study. In this review， the effects and mechanisms of Prunellae Spica in the treatment of various breast diseases were summarized， so as to provide a reference for further research on the wider clinical therapeutic effects of Prunella subtilis and its therapeutic effects on breast diseases.

Pharmacodynamics and Mechanism of Chaijin Sanjie Prescription on Rats with Mammary Gland Hyperplasia / 中国实验方剂学杂志

Objective:To investigate the pharmacodynamics and mechanism of Chaijin Sanjie prescription (CJSJP) on rat mammary gland hyperplasia, in order to provide experimental basis for the research and development of new Chinese medicine. Method:SD rat model of mammary gland hyperplasia was established through exogenous intramuscular injection with estrogen and progesterone. After successful establishment of the model, the rats were randomly divided into normal group, model group, and low, medium and high-dose CJSJP groups (3.13, 6.26, 12.52 g·kg-1) and Rupixiao (0.517 g·kg-1) group, with 9 rats in each group. After 28 days of administration, estradiol (E2), progesterone (P) and rolactin (PRL) were measured by radioimmunoassay, uterus and ovary coefficients were calculated; nipple diameter and breast histopathology were observed, estrogen receptor-α(ER-α) expression in mammary gland was measured by immunohistochemistry, and gonadotropin-releasing hormone (GnRH) and gonadotropin-releasing hormone receptor (GnRH-R) mRNA expressions in hypothalamus, pituitary were measured by Real-time PCR. ICR mice were randomly divided into normal group, low, medium and high-dose CJSJP groups (5.2,10.4,20.8 g·kg-1) and Luotongding group (0.038 6 g·kg-1) according to their body weight. Twelve mice in each group were given drugs for 7 days, and 0.6% acetic acid was injected intraperitoneally for 30 minutes after the last administration. The writhing times were observed within 15 minutes. Result:Compared with the normal group, the diameter of nipple was widened, serum E2 was significantly increased (Pα expression were increased in model group. compared with model group, the diameter of nipple was significantly decreased in high dose group of CJSJP (P2 was decreased in all dose groups of CJSJP, pathological score of breast hyperplasia was decreased in middle and high dose groups of CJSJP, GnRH mRNA in hypothalamus was decreased in all dose groups of CJSJP. The writhing times of mice in high dose group of CJSJP was decreased (PConclusion:Chaijin Sanjie prescription can improve the lesions of breast hyperplasia. The therapeutic mechanism may be related to the regulation of GnRH gene expression in hypothalamus and the decrease of estrogen receptor expression.

Changes of sex hormones in male patients with hyperthyroidism and hyperplasia of mammary glands / 国际检验医学杂志

Objective To investigate the effect of methimazole on male patients with hyperthyroidism and hyperplasia of mammary glands and its effect on sex hormone levels.Methods A total of 97 cases of hyper-thyroidism treated in the hospital from February 2014 to February 2017 were selected as the research subjects. These patients were divided into group A (patients with hyperthyroidism with mammary gland development , 33 cases) and group B (patients with hyperthyroidism ,64 cases) according to whether patients had the combi-nation of hyperplasia of mammary glands.Two groups of patients were treated with methimazole ,thyroid hor-mone levels and sex hormone levels before and after treatment were compared between the two groups.Results Before treatment ,there was no significant difference in the levels of free T 3 (FT3) ,free T4 (FT4) ,three io-dine thyroxine (T3) ,thyroxine (T4) ,and thyroid stimulating hormone (TSH) in the two groups (P＞0.05). After treatment ,the levels of FT3 ,FT4 ,T3 and T4 in both groups were significantly lower than those before treatment ,while the level of TSH was significantly higher than that before the treatment (P＜0.05).There was no significant difference in the levels of FT 3 ,FT4 ,T3 ,T4 and TSH between the two groups (P＞0.05). Before treatment ,the levels of total testosterone (T) ,luteinizing hormone (LH) ,prolactin (PRL) and estradi-ol (E2) in group A were significantly higher than those in the B group (P＜0.05).After treatment ,the levels of T ,LH ,PRL and E2 in group A were significantly lower than those before treatment (P＜0.05).After treat-ment ,the levels of T ,LH ,FSH ,PRL ,E2 ,P in the two groups were not statistically significant (P＞0.05). Conclusion Patients with hyperthyroidism combined with hyperplasia of mammary glands has a high level of sex hormones.After hyperthyroidism treatment ,thyroid hormone and sex hormone levels can be significantly changed into a better status.

Effect of Huiru Yizeng Granules on Hhyperplasia of Mammary Glandsin Rats with Hhyperprolactinemia / 医药导报

Objective To compare the effects of Huiru Yizeng granules Huiru yizeng original decoctin on hyperplasia of mammary glands with hyperprolactinemia in serum and pathological morphology of mammary glands tissue.Methods Seventy rats were randomly divided into 7 groups:normal control group,model control group,Huiru yizeng granules group,Huiru yizeng original decoctin group,Rubisanjie group,bromocriptine group and accessories group.After sucessfully modeling hyperplasia of mammary glandsin rats with hyperprolactinemia,Huiru yizeng granules group was administrated 17.22 g·kg-1·d-1,Huiru yizeng original decoctin group was administrated 20.08 g·kg-1·d-1,Rubi sanjie group was administrated 0.416 mg·kg-1·d-1,bromocriptine group was administrated 0.393 mg·kg-1·d-1.These groups were intragastric administration 2 mL every day for 30 consecutive days.The morphology of pathological tissue in mammary gland was observed by microscope.The levels of prolactin(PRL),progesterone(P),estradiol(E2) were determined by ELISA kit.Results Compared with the model group,Huiru yizeng granules group[PRL=(22.74±4.74) pg·mL-1,P=(46.91±2.85) ng·mL-1,E2=(99.96±9.61) pg·mL-1],Huiru yizeng original decoction group[PRL=(28.41±6.37) pg·mL-1,P=(43.91±4.17) ng·mL-1,E2=(105.02±3.05) pg·mL-1] and bromocriptine group[PRL=(23.58±4.10) pg·mL-1,P=(45.99±2.95) ng·mL-1,E2=(98.04±9.98) pg·mL-1]showed significant decrease in PRL,E2 levels,obvious increase in P(P<0.01).In Huiru yizeng granules group,Huiru yizeng original decoction group and bromocriptine group,PRL,P,and E2 returned to normal level after 30 days,and hyperplasia of mammary glands tissue had great ease.Huiru yizeng original decoction group was Ⅰ to Ⅱ hyperplasia,and Huiru yizeng granules group was 0 or Ⅰ hyperplasia.Compared with each other,the effect of Huiru yizeng granules on the mammary gland proliferation inhibition was superior to Huiru yizeng original decoction group.Conclusion On the treatment of hyperplasia of mammary glands and hyperprolactinemia,Huiru yizeng granules were better than Huiru yizeng original decoction.

Clinical Observation of Cutaneous Scraping and Cupping Therapy for Treatment of Hyperplasia of Mammary Glands / 广州中医药大学学报

Objective To evaluate the clinical efficacy of cutaneous scraping and cupping therapy for the treatment of hyperplasia of mammary glands. Methods Eighty patients with hyperplasia of mammary glands were randomized into test group(42 cases) and control group(38 cases). Both groups were given conventional basic treatment including psychological counseling and oral use of Chinese patent medicine, and the test group additionally received cutaneous scraping and cupping therapy on acupoints of Xuanji(CV21), Danzho ng (CV17), Jianjing(GB21), Kufang(ST14) , Tianzhong(SI11), Dazhui(GV14), and Ashi(focus of mammary mass and pain). The scores of pain visual analog scale(VAS) and World Health Organization Quality of Life Scale Brief Version(WHOQOL-BREF) were observed, and the clinical efficacy of the two groups was evaluated. Results (1) The total effective rate of the test group was 88.10%, and that of the control group was 65.79%, the inter-group difference being statistically significant (P0.05). Test group had better effect on improving VAS scores than the control group(P0.05). The control group showed no obvious changes in the scores of each dimension of WHOQOL-BREF before and after treatment(P>0.05). The inter-group comparison showed that the test group had better effect on improving the scores in physiological dimension of WHOQOL-BREF (P<0 . 05). Conclusion Cutaneous scraping and cupping therapy is effective on relieving pain and improving QOL of patients with hyperplasia of mammary glands.

Observations on the Therapeutic Effect of Acupoint Catgut Embedding on Breast Cystic Hyperplasia of Blood Stasis-phlegm Pattern / 上海针灸杂志

Objective To investigate the clinical efficacy of acupoint catgut embedding in treating breast cystic hyperplasia of blood stasis-phlegm pattern.Methods One hundred and three patients with breast cystic hyperplasia of blood stasis-phlegm pattern were randomly allocated to a treatment group of 53 cases and a control group of 50 cases. The treatment group received acupoint catgut embedding and the control group took Rupi Sanjie granules. The symptom/sign scores and the qi stagnation transformation score were recorded and sex hormone levels were measured in the two groups before and after 12 weeks of treatment. The clinical therapeutic effects were compared between the two groups.Results The cure rate and the total efficacy rate were 50.9% and 92.5%, respectively, in the treatment group and 46.0% and 90.0%, respectively, in the control group. There were no statistically significant differences in the cure rate and the total efficacy rate between the two groups (P>0.05). There were statistically significant post-treatment differences in the symptom/sign scores (pain, mass hardness, mass range, mass size and accompanying symptoms scores) between the two groups (P<0.05). At the three-month and six-month follow-ups, there were statistically significant differences in the pain, mass range and accompanying symptoms scores as compared with at the end of treatment in the treatment group, and between the treatment and control groups (P<0.05). There was a statistically significant pre-/post-treatment difference in the qi stagnation transformation score in the two groups (P<0.01,P<0.05). There was a statistically significant difference in the qi stagnation transformation score at the three-month and six-month follow-ups compared with at the end of treatment in the treatment group (P<0.05). There was a statistically significant difference in the qi stagnation transformation score between the treatment and control groups at the end of treatment and at the three-month and six-month follow-ups (P<0.05). There was a statistically significant pre-/post-treatment difference in the E2 content in the two groups (P<0.05). There were statistically significant pre-/post-treatment differences in the P and PRL contents in the treatment groups (P<0.05).Conclusion Acupoint catgut embedding is an effective way to treat breast cystic hyperplasia of blood stasis-phlegm pattern.

Therapeutic Observation of Mild Moxibustion for Hyperplasia of Mammary Glands in Female College Students of Different Constitutions / 上海针灸杂志

ObjectiveTo explore which body constitution responds the best in the treatment of hyperplasia of mammary glands with mild moxibustion, by differentiating the constitution following the self-rating scale for theClassification of Body Constitution of Traditional Chinese Medicine.MethodTotally 118 patients were intervened by mild moxibustion, majorly at Jianjing (GB21), but assisted by other points according to the nine constitution types (moderate, qi deficiency, blood stagnation, phlegm-dampness, qi stagnation, damp-heat, yin-deficiency, yang-deficiency, and specific constitutions). The mammary lump, pain in breast, and complicated symptoms were observed before and after intervention by following the self-comparison rules.ResultThere were no significant changes in mammary lumps in the 9 constitution types (P>0.05); the phlegm-dampness, qi-deficiency, blood stagnation, qi stagnation, and yang-deficiency constitutions showed significant improvements in the evaluation of breast pain and complicated symptoms (P0.05).ConclusionMild moxibustion can significantly improve the symptoms in some constitutions, but it seems that dry mild moxibustion is impossible to reverse the organic lesions and its efficacy is rather limited.

Therapeutic Observation of Acupoint Application plusXiao Pi Jianfor Hyperplasia of Mammary Glands / 上海针灸杂志

ObjectiveTo observe the clinical efficacy of acupoint application plusXiao Pi Jianin treating hyperplasia of mammary glands.MethodTotally 103 patients with hyperplasia of mammary glands were randomized into a treatment group of 52 cases and a control group of 51 cases. The treatment group was intervened by acupoint application plus oral administration ofXiao Pi Jian, while the control group was by oral administrationofXiao Pi Jianalone. The criteria for diagnosis and therapeutic evaluation of hyperplasia of mammary glands were adopted in the study.ResultThe total effective rate was 94.2% in the treatment group, significantly higher than 82.4% in the control group(P<0.05).ConclusionAcupoint application plusXiao Pi Jiancan produce a significant efficacy in treating hyperplasia of mammary glands.

Therapeutic Observation of Electroacupuncture plus Topical Laser Treatment for Hyperplasia of Mammary Glands / 上海针灸杂志

ObjectiveTo observe the clinical efficacy ofelectroacupunctureplustopical laser treatmentin treating hyperplasia of mammary glands.MethodFifty-eight patients with hyperplasia of mammary glands were randomized into a treatment group of 30 cases and a control group of 28 cases. The treatment group was intervened byelectroacupunctureplustopical laser treatment, while the controlgroup was by oral administration of Ru Zeng Ning tablets. The intervention began from 15 d prior to the menstrual period. After 3 successive treatment courses, the clinical efficacies were compared, and the efficacies in treating three different patterns of mammary glands hyperplasia were also compared, i.e. qi-stagnation due to liver depression, accumulation of phlegm and stagnation, and imbalance of the Thoroughfare and Conception Vessels.ResultAccording to the comprehensive efficacy evaluation, the total effective rate was 86.7% in the treatment group versus 57.1% in the control group, and the difference was statistically significant (P0.05).ConclusionElectroacupunctureplustopical laser treatmentis effective in treating hyperplasia of mammary glands, especially for the type of qi-stagnation due to liver depression.

Therapeutic Observation of Acupoint Application for Hyperplasia of Mammary Glands / 上海针灸杂志

Objective To observe the clinical efficacy of acupoint application and moving cupping plus photo ionization treatment apparatus in treating hyperplasia of mammary glands. Method Sixty patients with hyperplasia of mammary glands were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by acupoint application and moving cupping plus photo ionization treatment apparatus, while the control group was by oral administration of Tamoxifen and Ru Pi San Jie tablets. The breast pain and lump score was observed before and after 2-month treatment, and the clinical efficacy was compared. Result The breast pain and lump scores were significantly changed in both groups after intervention (P0.05). The total effective rate was 96.7% in the treatment group versus 86.7% in the control group, and the difference was statistically significant (P<0.05). Conclusion Acupoint application plus moving cupping plus photo ionization treatment is an effective method in treating hyperplasia of mammary glands, and it can ease or reduce breast pain, and improve or reduce the lump.

Expression of ER and PR in hyperplasia of mammary glands and breast cancer / 肿瘤研究与临床

Objective To investigate the expression of ER and PR in hyperplasia of mammary glands and breast cancer. Methods The expression of ER and PR in 68 hyperplasia of mammary glands cases and 168 breast cancer cases were quantified by immunohistochemical method. Results The expression of ER in breast cancer(30%) was significantly higher than that in hyperplasia of mammary glands(10 %)(P ＜0.05) and there was no significant difference in PR between them. The expression of ER in postmenopausal breast cancer was significantly higher than that in postmenopausal hyperplasia of mammary glands (P ＜0.05) and there was no significant difference in ER between two premenopausal groups. The expression of PR in invasive lobular carcinoma was significantly higher than that in invasive ductal carcinoma and other types (P=0.005).Conclusion The expression of ER and PR may identify the characteristics of patholobiology in breast disease.

Effect of Rujieping Capsule on the Expression of ER and PR of Mammary Tissue in Rats with Hyperplasia of Mammary Glands / 中国药房

OBJECTIVE:To discuss the effects of Rujieping capsule on estrogen receptor(ER) and progesteron receptor(PR) of pathological tissues in rats with hyperplasia of mammary glands.METHODS:The rats were injected i.m with Estradiol benzoate injection for 25 days followed by the injection of Progesterone injection i.m for 5 days to establish the model of hyperplasia of mammary glands.Which were treated with high and low doses of Rujieping capsule respectively for 30 days with tamoxifen as positive control.RESULTS:Both tamoxifen and high and low doses of Rujieping capsules could lessen the symptoms of red swelling of nipples or hyperplasia of mammary glands,lower the levels of E2 and PRL,enhance the level of progestone,lower the index of uterus,markedly reduce the number of layers of the ductal epithelial cells,reduce the levels of ER and PR in mammary glands,and suppress mammoplasia.CONCLUSION:Rujieping capsules is effective in the treatment of hyperplasia of mammary glands in rats by regulating the level of estrogen and improve pathomorphologic change of hyperplastic mammary glands.

Preparation and Quality Criteria of Ru'an Mixture / 中国药房

OBJECTIVE:To preparation Ru'an mixture,and establish its quality standard and observe its therapeutic efficacy.METHODS:Ru'an mixture was prepared with Radix Bupleuri and Ramulus Cinnamomi and Radix Angelicae Sinensis as raw material.Radix Bupleuri,Radix Paeoniae Alba,and Radix Angelicae Sinensis were identified by TLC and the content of Tanshinol was determined by HPLC.RESULTS:The TLC spots of Radix Bupleuri,Radix Paeoniae Alba,and Radix Angelicae Sinensis were all clear.The linear range of Tanshinol was 0.203～2.030?g(r=0.999 8).The total effect rate in Ru' CONCLUSION:Ru'an mixture is reasonable in preparation technique,controllable in quality.

Effects of Ruzengxiao on Rats with Hyperplasia of Mammary Glands / 中国药房

OBJECTIVE:To observe the efficacy of Ruzengxiao in the prevention and cure of hyperplasia of mammary glands in rats.METHODS:To investigate the therapeutic effect of drugs,the spayed rats were injected i.m with benzoic acid E2 0.8mg? kg-1 qd alt for 90 consecutive days,during the period between 20 and 30 days after modeling,these rats were injected with progesterone 4mg? kg-1 qd alt for 5 consecutive times;30d after modeling,the model animals with hyperplasia of mammary glands were randomized to groups to be treated intragastrically with 0.05% CMC-Na,Ruzengxiao or Tomoxi fen qd for 60d consecutively.Samples were taken at 24h after the last time of drug administration for determination of indexes.RESULTS:After treating the model animals for 2months,the following experimental results were obtained:for the spayed animals,after injection with estradiol(E2)for 3mo,their index of thymus decreased,indexes of uterus and adrenal glands increased significantly;levels of estrogen receptor(ER)in mammary tissues and serum E2 increased significantly,while the serum progesterone(P)deceased significantly,ratio of E2/testosterone(T)increased significantly;the numbers of lobules of mammary gland increased,hyperplasia of interstitial tissues appeared,secretion of acini increased,and the acini were highly distended,level of prolactin(PRL)increased,while levels of luteinizing hormone(LH)and follicle stimulating hormone(FSH)decreased,but no statistic difference was noted as compared with castration group(spayed animal without administration with E2 and P etc.);the E2-induced increase in indexes of uterus and adrenal gland was significantly inhibited after treatment with Tamoxifen or Ruzengxiao 20g? kg-1 for 2mo;Both Ruzengxiao(at different dosage)and Tomoxifen could significantly increase serum Testosterone level but decrease ratio of E2/T in rats with hyperplasia of mammary glands,showing significant differences as compared with model group.All drugs showed no obvious effect on serum levels of LH,FSH,PRL,P and E2 etc in rats with hyperplasia of mammary glands.CONCLUSION:The above results reveal that Ruzengxiao has satisfactory preventive and curative effects on model rats with hyperplasia of mammary glands.This mechanism might be related to the reduction of serum levels of E2,P and PRL,and the ratio of E2/T.

Effect of Tongru Sanpitang on Hyperplasia of Mammary Glands in Model Rats / 中国中医药信息杂志

Objective To research the mechanisms and the link of therapy and the optimal dosage of Tongru Sanpitang on hyperplasia of mammary glands in model rats. Methods Rats were randomly divided into 6 groups:negative contronl group, model group, Rupixiao Tablets group and Tongru Sanpitang low, middle, high dose group. Model was made with complex method of Progesterone and Estradiol benzoatehad injected into abdominal. In the middle of experiment, rats were given different drug respectively. Estradiol, follicle stimulating hormone, luteinizing hormone, the expression of estrogen receptor, weight of the ovary and uterus and visceral coefficient were determined. Results Follicle stimulating hormone, luteinizing hormone, the expression of estrogen receptor in medicine group decreased. The ovary and uterus in electron microscope, weight of organs and organ coefficient were improved. Conclusion Tongru Sanpitang may adjust reproductive endocrine function by decreasing sensitivity of mammary glands to estradiol and adjust whole function in the treatment of hyperplasia of mammary glands. There are dose-effect relationship.

A research about the influence of nursing intervention on the anxiety among patients with hyperplasia of mammary glands / 中国实用护理杂志

Objectivestudy the influence of nursing intervention on the anxiety among patients with hyperplasia of mammary glands. Methods Divided 200 patients with hyperplasia of mammary glands into the experimental group and the control group, there were 100 cases in the each group. The nursing intervention was used in the experimental group before the examination and the routine nursing cares were used in the control group. Using the HAMA scale to evaluated the anxiety of the two groups. Results The anxiety in the experimental group was significant better than that of in the control group,P

Effect of Acupuncture on Estrogen,Estrogen Receptor and Estrogen Signal Transduction in Rat Model of Hyperplasia of Mammary Glands / 广州中医药大学学报

0.05).After treatment,the nipple height was increased in the model group(P